Journal: Journal of Pain Research

Article Title: Effects and Mechanisms of Wrist-Ankle Acupuncture on Inflammatory Pain in the Rat Gastrocnemius Muscle

doi: 10.2147/JPR.S605568

Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Article Snippet: Complete freund’s adjuvant (CFA) (Sigma, USA, F5881), trichloroethanol (Aibei, Nanjing, CN, M2820), acupuncture needles (Jiajian Medical, CN), TENS-WAA equipment (Shanghai MicroPort Scientific Co, CN), Von Frey hairs mechanical stimulation needles (Yuyan Instrument, CN), Von Frey test cage (Yuyan Instrument, CN), Rat IL-6 ELISA Kit (Servicebio, CN, GER0001-96T), Rat IL-1β ELISA Kit (Servicebio, CN, GER0002-96T), Rat TNF-α ELISA Kit (Servicebio, CN, GER0004-96T), Rat TGF-β1 ELISA Kit (Servicebio, CN, GER0051-96T), BCA Protein Assay Kit (Servicebio, CN, G2026-1000T), TRIzol ® reagent (China, Servicebio, CN, G3013) SweScript All-in-One SuperMix for qPCR (Servicebio, CN, G3337), Blue SYBR Green qPCR Master Mix (Servicebio, CN, G3326).

Techniques:

Journal: Bioactive Materials

Article Title: Precisely regulated physically-crosslinked carriers enable synergetic release of bioactive factors for MSC-mediated cartilage regeneration

doi: 10.1016/j.bioactmat.2026.01.009

Figure Lengend Snippet: In vivo chondrogenesis of hydrogels incorporated with different combinations of PSF and KSF. (a) Alcian blue and Safranin O staining of different combinations after 21-day implantation. G1: 5 % β-sheet PSF +5 % β-sheet KSF; G2: 15 % β-sheet PSF +30 % β-sheet KSF; G3: 30 % β-sheet PSF +40 % β-sheet KSF; G4: 40 % β-sheet PSF +50 % β-sheet KSF. Scale bar = 200 μm. (b–d) Quantitative analysis of IL-1β, IL-6 and TNF-α surrounding defect cartilage 1 week and 3 weeks after operation. (e, f) Macroscopic and MRI observations of rat femoral condyles at week 6 and 12. Red circles and red arrows show the original defect zone under macroscope and MRI respectively. Scale bar = 1 mm. (g) Relative ratio of average optical density (versus G1) that shows the staining intensity of Alcian blue and Safranin O staining. (h) ICRS scoring of macroscopic evaluations. (i) The repaired cartilage using a nanoindentation instrument. Scale bar = 1 cm. (j, k) Reduced modulus and hardness of the regenerated cartilage. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: The suspension was centrifuged at 10,000 rpm for 10 min at 4 °C, and the supernatant was collected, and Elisa assay was performed following the manufacturer's instructions for rat IL-1β, IL-6, and TNF-α ELISA kits (Elabscience, China).

Techniques: In Vivo, Staining

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Concentration Assay, Control

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Derivative Assay, Concentration Assay

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Derivative Assay, Concentration Assay

Journal: iScience

Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

doi: 10.1016/j.isci.2026.115059

Figure Lengend Snippet: Prostaglandin dysregulation and inflammatory response in the RPP model (A–C) Uterine PGF 2α (A), PGE 2 (B), and PGF 2α /PGE 2 ratio (C) on days 12 and 24 of RPP modeling ( n = 3). (D) The protein levels of COX-2 in the uterine tissue on days 12 and 24 of RPP modeling ( n = 3). (E–G) The levels of IL-1β (E), IL-6 (F), and TNF-α (G) in the serum on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, one-way ANOVA.

Article Snippet: Rat IL-1β ELISA Kit , Elabscience , Cat# E-EL-R0012.

Techniques: